Fibroblast culture

Test #:

975

Turnaround Time:

3-4 weeks

Specimen Requirements:

Specimens should be shipped at room temperature in a leak-proof, rigid container with overnight delivery. To maximize the likelihood the specimen will grow in culture, we recommend using an Allele-supplied kit or shipping label to ensure the sample arrives in the lab the day after collection. We also ask for specimens to be collected under sterile conditions and shipped in RPMI or sterile saline.

• Skin biopsy: 2x2 mm to 3x3 mm (Order a kit)

CPT Codes:

88233 x1

Ordering Requirements:

• Fibroblast Culturing and Extraction Requisition Form
• Please see Fibroblast Culturing and DNA Extraction page for details about this process.

Test Description:

Fibroblast culturing for DNA-based testing from skin biopsies.

The purpose of fibroblast culturing at Allele Diagnostics is to obtain DNA from this tissue type for germline testing performed at another laboratory, as many sequencing laboratories do not have culturing capabilities. Our laboratory will extract from the skin biopsy then culture from the remaining tissue; our priority is to obtain DNA from cultures. Once we have DNA obtained from cultures, we will forward the DNA on to the laboratory performing sequencing (or other testing). We will maintain cultures as a backup until testing is complete or until cultures are no longer healthy. Allele will not issue a report, as no testing is performed in our laboratory. Please note: Skin biopsies are very fragile specimens. Tissue culture success can depend on numerous factors including age of the specimen, age of the patient, shipping conditions, etc.

If tissue culture is required on a tissue type other than skin biopsies, please contact our laboratory at 844-255-3532 or email at info@allelediagnostics.com to discuss with our team prior to obtaining a sample.